A cost effective alternative method to ddRADseq library construction during size selection

Next generation sequencing (NGS) technologies constitute the most powerful scientific advance of 21st century with a promise fast and cost effective data in biology. Yet, up to date NGS studies remain often limited laboratories established resources. In present study, we employed construction ddRADseq library by using routine lab consumables (agarose gel electrophoresis: AGE thereafter) compared high-tech (paramagnetic beads) during size selection. The was constructed for selected based on universally used paramagnetic beads, while remaining aliquot as template assess feasibility labs Both libraries were optimised 15 PCR cycles indicating similarity intensity. Post-PCR quantification comparable (~10 ng.µL-1). Size distribution assessment revealed cleaner pick at manually AGE. Similarly, intercalating agent Qubit confirmed quantity similar (>3 Although being more time consuming due pre-electrophoresis preparations, serial wash staining steps, is achievable provided supply adaptors primers initial wet-lab work. These results manifest